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Current Issue 2020

  • Pages: 115-119 Vol-17 No-2

    EVALUATING THE STATUS OF VITAMIN 25(OH) D LEVELS AMONG FEMALES OF ALL AGE GROUPS IN KARACHI, PAKISTAN (VITAMIN D LEVELS AMONG FEMALES IN KARACHI)

    Corresponding Author

    Tehseen Fatima Zeb

    tehseen.fatima@duhs.edu.pk,
  • Abstract

    Authors: 1Farrukh Abu Hazim, 2Rafat Amin, 2Urooj Ishrat, 4Neha Baqai, 2Tehseen Fatima Zeb*

    Objective: Vitamin D plays an imperative role in growth, metabolism and reproduction. Low levels of this vitamin D, are highly prevalent globally and leads to various disorders besides different skeletal deformities. Women of different reproductive ages are more prone towards developing bone diseases as of low vitamin D levels. The study focuses to evaluate the prevalence of vitamin D deficiencies in females of different ages in Karachi, Pakistan.
    Methods: Serum vitamin 25(OH) D levels of 1035 female were measured by electro-chemiluminiscence immuno assay. The data was analyzed through SPSS version 16.
    Results: Among 1035 participants of different reproductive ages, 26.1% (n= 270) showed severe vitamin D deficiency, 44.2% (n=458) displayed mild to moderate Vitamin D deficiency while 29.7% (n=307) were having normal levels of serum vitamin D.
    Discussion and conclusion: 70.4 % of our studied population had lower levels of vitamin D representing that majority of Pakistani women have vitamin D Deficiency. Since hypovitaminosis is a grim public health concern, strong recommendation of health education and vitamin D supplementations for the female population is proposed.

    Keywords: Vitamin 25 (OH) D levels, Female population, different age, Karachi, hypovitaminosis

  • Pages: 107-113 Vol-17 No-2

    AN OVERVIEW ON SUITABILITY OF ECO-FRIENDLY IPM APPROACH FOR MANAGEMENT OF BT-RESISTANCE IN PINK BOLLWORM ON COTTON

    Corresponding Author

    Muhammad Shahid Iqbal

    shahidkooria@gmail.com
  • Abstract

    Authors: Rao Ahsan Ayaz1,2, Muhammad Rafiq Shahid1 , Saghir Ahmad1 , Muhammad Javaid2 and Muhammad Shahid Iqbal1*

    Pink Bollworm (Pectinophora gossypiella) member of the family Gelechiidae is known to be among the most destructive insect pest of cotton, causing huge damage to the cotton seed as well as heavy loss in fiber quality of the crop. Its larvae are active feeder of the reproductive parts of cotton and conceal in the damaged bolls. Large scale cultivation of transgenic cotton that produces Cry1Ac resulted in development of resistance in pink bollworm against Cry1Ac. A single control tactic even chemical control remains ineffective for their management and all possible tactics are integrated for their effective control. For its monitoring Sex pheromone traps, PB Ropes and light traps are installed. Growing of non Bt refugia also play an effective role for the management of pink bollworm. Keeping in view, the importance of Integrated Pest Management (IPM) is an ecofriendly and effective technique as compared with the use of conventional insecticidal technique. /uploads/2020/Vol-17/No-2/PJBT-VOL-17-NO-2-OF-YEAR-2020 (8).pdf

    Keywords: Pectinophora gossypiella: Bt resistance: Management Strategies

  • Pages: 101-105 Vol-17 No-2

    PUBLIC AWARENESS INFORMATION ON HUMAN VIRUSES AND CORONAVIRUS (COVID-19): I. CHARACTERISTICS AND PROTECTION

    Corresponding Author

    Atef Shoukry Sadik

    atef_sadik@yahoo.com & atef_sadik@agr.asu.edu.eg
  • Abstract

    Authors: Atef Shoukry Sadik

    This research can be considered as simple awareness information for the people with limited education in Egypt as well as the world in addition to specialists. It also allows decision makers in the World Health Organization and other countries to devise appropriate strategies to respond to any epidemic such as the Coronavirus and others that may arise in the future. This article has dealt with a simple definition of viruses and then focus on the characteristics of human viruses in a simplified manner, whether their ability to enter the family of the host, how to influence it, the concept of the incubation period, the role of the immune system in the long of this period, and the prevalence of an epidemic including viruses. This is in addition to the symptoms of coronavirus infection, and prevention methods. Finally, the study pays an attention on the most nutrition system and factors that positively affect the immune system and its strengthening in a way that allows humans to confront viral infection.

    Keywords: Viruses, coronavirus infection, symptoms, incubation period, immune system, nutrition, controlling strategies.

  • Pages: 93-99 Vol-17 No-2

    BIBLIOMETRIC ANALYSIS OF THE PAKISTAN JOURNAL OF BIOTECHNOLOGY

    Corresponding Author

    Waseem Hassan

    waseem_anw@yahoo.com
  • Abstract

    Authors: Waseem Hassan a, , Haseeb Hassan b and Mehreen Zafar a

    This is the first bibliometric report about the Pakistan journal of biotechnology (PJBt), which was launched in 2004. Total number of publications was found to be 825. Statistically, a random yearly growth rate was observed (varied per year) and the doubling time was found to be 4.7 (years). From 2008 to 2017, the data of 501 publications was retrieved from Scopus. Precisely, 1224 authors, 714 institutions and 29 countries were directly involved in (501) research publications. In authors category, Prof. Dahot M.U. was found with highest number of publications i.e. sixteen (16). Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan is the top university with (29) publications. While, India is the top ranked country with maximum (271) number of publications. We also provided the list of top ten most cited documents. Last but not the least, the detail graphical maps are provided for the co-words analysis of titles, abstracts and all keywords of the manuscripts (Vosviewer analysis). This will significantly help to describe the overall theme of the journal.

    Keywords: Scopus, PJBt and Bibliometry

  • Pages: 85-91 Vol-17 No-2

    ANTIHYPERTENSIVE PEPTIDES PRODUCED BY INDIGENOUS LACTIC ACID BACTERIA FROM DADIH ORIGIN

    Corresponding Author

    Chandra Utami Wirawati

    cutami@polinela.ac.id
  • Abstract

    Authors: Chandra Utami Wirawati1*, Dwi Eva Nirmagustina and Yatim Rahayu Widodo

    In this study the capability of Lactobacillus plantarum ssp. plantarum DG17 and Lactococcus lactis ssp. lactis DK12 isolated from dadih origin was considered to generate antihypertensive peptides in reconstituted skim milk (RSM) medium followed bymeasuringangiotensin converting enzyme (ACE) inhibitor activity, protein profile and protein sequence. Theisolates showed high ACE inhibitor abilities i.e. 60.796.2% and 61.987.8%, with IC50 values of 439.9 g/mL and 442.2 g/mL respectively. Two smaller molecular weight bands ranging in size from 12 to 14 kDa were recorded in peptides profile of these isolates and assumed as antihypertensive peptides. The peptides sequence with molecule weight less than 3.0kDa were identified by LC Nano/MS and then compared withthe peptide database (Milk Bioactive Peptide Databaseand BIOPEP-UWM).One of the peptide sequence (VVVPPF) generated from Lactobacillus plantarum ssp. plantarumDG17had high ACE inhibitor activity due to the presence of F residue at the C terminal and V residue at the N terminal. The experimental result indicated that Lactococcus lactis ssp. Lactis DK12 and Lactobacillus plantarum ssp. plantarum DG17 isolates were potential developed into a starter culture in functional fermented milk.

    Keywords: : Lactobacillus plantarum, Lactococcus lactis, ACE inhibitor, antihypertensive peptides

  • Pages: 79-84 Vol-17 No-2

    EVALUATION OF DIFFERENT FUNGICIDES, BOTANICAL EXTRACTS AND BIOCONTROL AGENTS AGAINST ALTERNARIA ALTERNATA THE CAUSAL AGENT OF LEAF SPOT IN SPINACH

    Corresponding Author

    Manzoor Ali Abro

    mmanzoorabro@hotmail.com
  • Abstract

    Authors: Aziz Ullah Kakar1 , Manzoor Ali Abro1* , Ghulam Hussain Jatoi1 , Mir Shahbaz Ali Talpur1 , Waseem Ali Soomro1 , Suman Qazi1 , Mohammad Dawood2 and Adnan Baloch2

    Alternaria disease is among the most important diseases of Spinach throughout the world. They affect primarily the leaves. Alternaria spp. is major plant pathogens, which cause at least 20% of agricultural spoilage most severe losses may reach up to 80%.This study was carried out to study the in-vitro efficacy of different fungicides, botanical extracts and bio-control agent against leaf spot disease of spinach caused by Alternaria alternate. Cabrio top showed minimum mycelial colony growth (23, 21 and 17 mm) at various concentrations 100, 200 and 300 ppm, respectively, followed by Topsin-M (44.5, 33 and 19 mm), Melodyduo (33, 26.5 and 22.5 mm), Prevail (43.5, 37 and 22.5 mm) and Antracol (41, 33 and 24 mm) at various concentrations 100, 200 and 300 ppm, respectively. However, in control, the mycelial colony growth of A. alternata causing leaf spot disease was recorded as 90 mm at 100, 200 and 300 ppm concentrations. Minimum mycelia colony growth (43, 35 and 15 mm) was recorded at 5, 10 and 15% concentration for Aak followed by Garlic (43.5, 33 and 20 mm), Neem (49, 38.5 and 23 mm), Onion (57.4, 50.25 and 27.65 mm) and Eucalyptus (57.9, 51.4 and 33.45 mm) and maximum mycelial colony growth (90.00 mm) were recorded in control. Minimum mycelial colony growth of A. alternata was observed for Neurospora spp. (47.00 mm) followed by Hypocrea sp. (48.00 mm), Lasodiplodia sp. (49.5 mm), Fusarium sp. (57.5 mm) and Chactomium sp. (57.5 mm). Maximum mycelial colony growth (90 mm) was recorded in control.

    Keywords: Alternaria alternate, Biocontrol, Botanical extracts, Fungicides, leaf spot, Spinach

  • Pages: 71-77 Vol-17 No-2

    IDENTIFICATION OF VALUABLE TRAITS THROUGH MOLECULAR AND MORPHOLOGICAL MARKERS IN DIPLOID WHEAT

    Corresponding Author

    Zareen Sarfraz

    zskpbg@hotmail.com
  • Abstract

    Authors: Zareen Sarfraz1* , Mohammad Maroof Shah1 , Muhammad Shahid Iqbal2 , Mian Faisal Nazir2 , Syeda Akash Fatima

    Bread wheat (Triticum aestivum L.) harbors hexaploid genome with a complex of three diploid genomes (A, B and D). Major goals of todays research in the wheat world count basically on improvement of grain quality and high yielding genotypes. It is relatively easier to target its diploid lineages for identification of high yielding genomic segments as compared to hexaploid with least polymorphism. The current study was aimed at the detection of genes/QTLs of high economic interest on A genome of diploid progenitors. Segregating population from cross of Triticum monococcum and Triticum boeoticum was utilized for the purpose. 98 RILs along with parents were analyzed for various quantitative and qualitative traits. The molecular data obtained from RAPD markers was analyzed along with morphological traits data to identify association among them. Single factor ANOVA gave association (?= 0.01) of days to booting, days to heading and days to flowering with plant type and leaf orientation, flag leaf width with crown color, number of spikes per experimental unit with leaf orientation, 1000 kernel weight with aleurone color and RAPD-11 (locus b).

    Keywords: A-genome donor, Wheat, RILs, RAPDs, QTLs

  • Pages: 63-70 Vol-17 No-2

    EFFECT OF RATIO OF BEES WAX AND CARNAUBA WAX IN MIXED WAX ON RESPIRATION RATE, WEIGHT LOSS, FRUIT DECAY AND CHEMICAL QUALITY OF VIETNAMESE PASSION FRUITS DURING LOW TEMPERATURE STORAGE

    Corresponding Author

    Nguyen Sang

    nguyensang78vn@gmail.com
  • Abstract

    Authors: Nguyen Sang* and Le Ha Hai

    This research aims at studying the impacts of ratio of bees wax and carnauba wax in mixed wax (MW) on weight loss, respiration rate, fruit decay and the chemical quality of the Vietnamese fresh purple passion fruit in storage period at low temperatures. Firstly, the MW1, MW2 and MW3 were made from bees wax and carnauba wax with 2 to 1, 1.5 to 1 and 1 to 1 ratios respectively at concentration of 8%. After that, passion fruits were coated in MW1, MW2 and MW3 for thirty seconds and dried for eight hours at room temperatures. After that, they were stored at 51C for 42 days. The control was uncoated fruits. The rate of respiration, weight loss and fruit decay and total microorganism, chemical quality, content of total soluble solids (TSS), total titratable acidity and content of vitamin C were monitored during the storage period. According to the results, the MW1 treatment had the lowest rate of respiration rate, fruit weight loss, fruit decay and total microorganisms for a 42 day duration in storage. Besides, this control and all treatments could preserve the chemical quality of the passion fruit in terms of TSS content, total titratable acidity and content of vitamin C during storage time with insignificant difference (P?0.05).

    Keywords: Purple passion fruit, bees wax, carnauba wax, mixed wax coating, respiration rate, weight loss, fruit decay.

  • Pages: 53-61 Vol-17 No-2

    Citrus limetta (Musambi) PEELS: AN EFFICIENT CHEAP SUBSTRATE FOR TANNASE PRODUCTION IN SOLID STATE FERMENTATION

    Corresponding Author

    Muhammad Irfan

    irfan.biotechnologist@gmail.com
  • Abstract

    Authors: Hafiz Abdullah Shakir1 , Kiran Fatima1 , Muhammad Irfan2*, Muhammad Khan1 , Shaukat Ali3 , Saba Saeed1 , Javed Iqbal Qazi1

    The present study mainly executed to optimize various factors for tannase production employing agro-industrial waste. Ten bacterial strains isolated from fish gut were revived on nutrient agar and then cultivated on tannin-agar plates for holozone and tannase assay. Of all, Klebsiella oxytoca was selected due to highest enzyme production (24.52 U/ml) potential among other strains. The medium ingredients (musambi peels (2%), trypton (0.2%), potassium nitrate (0.05%) and potassium chloride (0.1%) was selected employing one variable at a time approach. Optimized culture parameters such as initial medium pH of 3, incubation temperature of 30C, with 1% inoculum size and 24 h of fermentation period yielded highest enzyme production. Partial characterization of the crude enzyme revealed best enzyme activity at pH 5 and 40C. The optimum time of enzymatic action was 30 minutes with substrate concentration of 1.5%. These finding could be utilized for further scale up studies.

    Keywords: Fish gut, Klebsiella oxytoca, tannase, optimization, SSF

  • Pages: 49-52 Vol-17 No-1

    SHORT COMMUNICATION : COMPARISON OF ANTHROPOMETRIC INDICATORS IN ANEMIC AND NON-ANEMIC

    Corresponding Author

    Jamshed Warsi

    jamshed.warsi@usindh.edu.pk
  • Abstract

    Authors: Jamshed Warsi, Benazir Mahar

    Background: The anemia is the one of the biggest burden of diseases worldwide, peculiarly in developing world, it is more commonly found in women as compared to men. The anthropometric indicators like BMI, waist circumference or waist to hip ratio has been associated with cardiovascular risk factors, diabetes, blood pressure and cholesterol profile. The current literature survey reveals that BMI could be the possible anemic indictor, however a comprehensive study pinpointing on anemia and its anthropometric indicators is not available yet, especially in young girls. The present work thus focuses on whether the anthropometric indicators could be the potential indicators of anemia or not.
    Methodology: A case control was performed through a random sampling method for the time period of (04) four months. The sample comprised of 86 females. The complete blood count (CBC) was measured in aseptic condition. Anthropometric indices were measured and analyzed statistically.
    Results: <> Weight, waist circumference (WC), BMI (Body mass index), WHR (waist to hip ratio) and WHtR (Waist to height ratio) were observed significantly reduced (P=0.06, P=0.006, P= 0.02, P= 0.007, P= 0.0007) in anemic (50.86kg 12.9, 71.13cm 16.49, 20.85Kg/m2 5.16, 0.77 0.05, 0.440.07) as compared to non anemic females (53.65kg 8.43, 92.18cm 11.16, 23.40 Kg/m2 3.49, 0.83 0.16, 0.49 0.06). Conclusion: The anthropometric indices were observed significantly decreased in anemic as compared to non-anemic.

    Keywords: BMI, Waist to hip ratio (WHR), waist circumference (WC), anemia

  • Pages: 41-47 Vol-17 No-1

    BIOLOGY AND FEEDING POTENTIAL OF 7-SPOTTED BEETLE, COCCINELLA SEPTEMPUNCTATA (LINNAEUS) ON BERSEEM APHID, THERIOAPHIS TRIFOLII (MONELL) IN LABORATORY

    Corresponding Author

    Shah Nawaz Khuhro

    snkhuhro@gmail.com
  • Abstract

    Authors: Attaullah Rind Baloach1, Shah Nawaz Khuhro, Mohammad Farooque Hassan, Wazir Ali Metlo and Sultan Ahmed Maitlo

    The present experiment was carried out on Biology and feeding potential of 7-spotted beetle, Coccinella septempunctata L. on berseem aphid, Therioaphis trifolii (Monell in the Biological Control Laboratory, Central Cotton Research Institute Sakrand during 2015. For this purpose, adult beetles reared in the laboratory at constant temperature and aphid collected from different crops from vicinity of Central Cotton Research Institute Sakrand were given to lady beetle for feeding. The pre-copulation, copulation and post-copulation periods were recorded as (4.40.69),(51.54.37) and (4.01.05) minutes, respectively. The pre-oviposion, oviposition and post-oviposition periods were averaged as 4.70.30, 36.64.88 and 5.01.69 days, respectively. The female deposited an average of 599.1109.83 eggs during her life time in 30 days. Egg incubation period was noted 4.00.94 days with 90.35% hatching and mortality 17.56%. The larval and pupal durations were 12 and 5.2 days, respectively. The mean pupation rate % was 69.4979.23 and the larval cannibalism rate was 30.4979.23. The average number of pupae observed were 20.3 4.47, while the male emergence was 37.47 13.12 %) and the female emergence was 43.98 8.24%). The result showed consumption rate of 1st, 2nd, 3rd and 4th instar larvae of C. septempunctata (22.04.12, 56.85.26, 143.85.93 and 289.69.65 aphids/day) on T. trifolii, respectively. In the same way, consumption rate of adult female (77.418.84/day) and male (48.946.29 /day) was on T. trifolii.

    Keywords: Biology, Feeding Potential, 7-spotted Ladybird beetle, and Berseem aphid

  • Pages: 33-40 Vol-17 No-1

    DETECTION OF CASP-5 GENE AS INFLAMMATORY FACTOR IN IRAQI PATIENTS WITH PSEUDOMONAS AERUGINOSA INFECTIONS

    Corresponding Author

    Ali Hussein Alwan

    dralialameri587@gmail.com
  • Abstract

    Authors: Ali Hussein Alwan

    Pseudomonas aeruginosa is define as one of an aerobic Gram-negative bacteria that considered as one of most problematic nosocomial emerged pathogens. This study was performed on 50 isolates and blood patients. Definitive diagnosis of positive P. aeruginosa isolates based on the characteristic examination, the phenotypic of colonies and microscopic morphology which grown in media also biochemical tests for diagnosis of isolates Fifty isolates identified as Pseudomonas aeruginosa, the percentage of isolates according to clinical sources were 19(38%) from burn, 12 (24%) from urine,3(6%) from ear swabs,10(20%) from sputum and 6(12%) from wounds. The diagnosis was conducted and confirmed by using API20E the result of it is 47(94%). The results showed isolates a different ability to resist antibiotics. Amoxicillin- clavulanic acid, Trimethoprim-sulfamethoxazole and Erythromycin were the effectiveness antibiotic against P. aeruginosa 100%.The ability of biofilm formation as a virulence factor, Micro-titer plate method (MTP) was used, results revealed that (46) isolates (92%) of P. aeruginosa have the ability to form biofilm by MTP method. Genotypic detection was done by conventional PCR for 16SrDNA gene by using specific primer (pa-ss) 50(100%) to detect the virulence gene (Flic gene) which is virulence factor gene in p. aeruginosa responsible for activation of caspase-1enzyme, the result showed 45(90%) positive. all the blood sample the results were positive %100 . Sequencing for 20 casp-5 PCR product to detect variations in gene of Iraqi population and the results showed 20 sample from 20 have a genetic variation, heterozygous nucleotide.

    Keywords: Pseudomonas aeruginosa, Flagillin, flic and Casp-5 gene.

  • Pages: 25-31 Vol-17 No-1

    EVALUATION OF DIFFERENT FUNGICIDES, BOTANICAL EXTRACTS AND BIOCONTROL AGENTS AGAINST PENICILLIUM EXPANSUM THE CAUSAL AGENT OF BLUE MOLD OF APPLE

    Corresponding Author

    Manzoor Ahmed Reki

    sau4434@gmail.com
  • Abstract

    Authors: Manzoor Ahmed Reki, Manzoor Ali abro, Ghulam Hussain Jatoi, Munwar Ali Gadhi, Shar Muhammad and Amir Ali

    Efficacy of various fungicides, their botanical extracts and bio-control agents against Penicillium expansum, the agent which cause blue mold of apple. Incidence of disease was recorded in Hyderabad and Tandojam. Various fungicides like Topsin-M, Melody Duo, Antracol, Cabriotop and Alliette at the different doses i.e. 100, 200 and 300 ppm, their botanical extracts like Ginger, Neem, Eucalyptus, Garlic and Onion, it was carried out at different doses i.e. 5, 10 and 15% by food poisoning method, their Pathogenicity test which was also performed to see the severity of disease in apples. The results showed that maximum disease incidence 53.45% was observed from Hyderabad and 46.55% was observed from Tandojam. It was observed that the disc method of inoculation showed a higher percentage (93.44%) as compared to the injection method of inoculation 65.55%. The growth of minimum linear colony of Penicillium expansum were observed for Topsin-M as 39.33, 32.00 and 25.00 mm respectively followed by Antracol 42.66, 35.00 and 30, Melody Duo 40.66, 35.66 and 30.00, Alliete 44.66, 36.00 and 31.00 and Cabriotop 46.33, 40.00 and 33.66. The growth of linear colony Penicillium expansum was observed as 41.00, 35.00and 27.66 mm for Ginger respectively followed by Neem 45.00, 38.00 and 33.66, Eucalyptus 48.00, 41.00 and 35.33, Garlic 52.33, 45.33 and 39.66) and Onion 61.66, 52.00 and 46.00. The linear colony growth of Penicillium expansum was minimum to observed for Guignardia sp. 29.66 mm, followed by Chaetomium sp. 35.33mm, Hypoxylon sp. 35.66mm , Fusarium sp. 40.66mm, Trichoderma sp. 47.66mm.

    Keywords: Apple, Blue Mold, Fungicides, Botanical Extracts, Biocontrol, Penicillium Expansum

  • Pages: 17-23 Vol-17 No-1

    THE PROTECTIVE EFFECT OF DAFLON AGAINST THE RENAL-HEPATIC TOXICITY

    Corresponding Author

    Nael Mohammed Sarheed

    Nael.serheed@mu.edu.iq
  • Abstract

    Authors: Nael Mohammed Sarheed and Safa Azher Al-khalidy

    Methotrexate (MTX) is considered a folic acid antagonist, used frequently in the treatment of various types of diseases and has had a major role in the treatment of inflammation and suppressive material for immunity in Non tumor diseases like acute arthritis and psoriasis. This study was designed to investigate the effect of methotrexate and pre-treatment with daflon in attenuation of its development for renal-hepatic toxicity. Twenty-four healthy, local, domestic rabbits were randomized divided into 4 groups: group 1 (control), group 2 (20mg\kg MTX group), group 3 (Daflon 100 + MTX) and group 4 (Daflon 200 + MTX). At the end of experiment, blood samples were collected from the heart then the liver and kidney tissues were excised. Plasma level of S.ALT, S.AST, ALP, total protein, bilirubin, urea, creatinine and oxidation parameters (GSH, and MDA) were measured. The Results show that the Compared with the control group, levels of S.ALT, S.AST, ALP, urea, creatinine and MDA were increased (p<0.05), while total protein and GSH were decreased (p<0.05) in MTX group with deterioration of liver and kidney tissues. While group 3 (Daflon 100 + MTX) and group 4 (Daflon 200 + MTX) showed decreased levels of S.ALT, S.AST, ALP, urea, creatinine and MDA while total protein and GSH were increased (p<0.05) in as compared with MTX group with improvement of liver and kidney structures. Conclusions: The results of the present study reveal that daflon has been shown to attenuate MTX induced hepato-renal toxicity.

    Keywords: Methotrexate, hepatotoxicity, renal toxicity, daflon, oxidative stress.

  • Pages: 9-15 Vol-17 No-1

    EVALUATION OF THE ANTIBACTERIAL ACTIVITY OF SOME MEDICINAL PLANTS EXTRACT AGAINST SOME HUMAN PATHOGENIC BACTERIA

    Corresponding Author

    Sayed Abdullah

    sabdullah.msib05asab@asab.nust.edu.pk
  • Abstract

    Authors: Anees Ahmad and Sayed Abdullah

    The need of effective and economical antibiotics and antimicrobials has led to search for novel sources such as medicinal plants, fungi, archaea etc. In the present study, Daphne mucronata and Mallotus philippensis methanolic extracts were screened as antibacterial agents compared to some antibiotics against eight local isolates of human pathogenic bacteria. The susceptibility test was performed through well diffusion and disc diffusion method. Results reported that D. mucronata showed significant antibacterial activity against all the selected strains. The highest zones of inhibition of D. mucronata seed extract were recorded against, Providencia stuartii (20mm), Brucella abortus (19.3mm), Proteus vulgaris (19.3mm), Klebsiella pneumonia (19mm), followed by Pseudomonas aeruginosa (19mm), Enterobacter sakazaki (18.3mm), Escherichia coli (16.6mm) and Staphylococcus aureus (16.6mm). D. mucronata seed extract was reported as the most active against B. abortus (19.3mm), E. sakazaki (18.3mm), E. coli (16.6mm) and K. pneumoniae (19mm) when compared to Cefepime (8.1mm) and Ciprofloxacin (14.2mm). However, D. mucronata methanolic seed extract showed about similar antibacterial activity against P. vulgaris (19.3mm), P. stuartii (20mm), P. aeruginosa (19mm) and S. aureus (18.7mm), compared to the following antibiotics Cefepime (19.3mm) Ciprofloxacin (21mm), Moxifloxacin (21mm) and Ceftriaxone (20mm). On the other hand, M. philippensis showed insignificant antibacterial activity against the selected bacterial strains, but not significant compared to selected antibiotics. As conclusion, D. mucronata extract was considered as high potential antibacterial and recommend to be used as novel and economical source of effective antibacterial agent to combat against hu man patho genic ba cteria.

    Keywords: Antibiotics activity; Daphne mucronata; Mallotus philippensis; Human Pathogenic bacteria; Antibiotics; medicinal Plants

  • Pages: 1-8 Vol-17 No-1

    ASSESSMENT OF PREGNANT WOMENS KNOWLEDGE ABOUT ANTENATAL CARE DURING PREGNANCY AT PRIMARY HEALTH CARE CENTERS IN KIRKUK CITY

    Corresponding Author

    Iman Salman Hassan

    iman1i@yahoo.com
  • Abstract

    Authors: Iman Salman Hassan, Hanaa A. Omer

    The present study was designed to evaluate pregnant women's practices about antenatal care during pregnancy in primary health care centers in Kirkuk City after implementation of program. A quasi-experimental design was used in the present study with the application of a pre-test/ post-test approach for the study group assessment, The results show the distribution of studied socio-demographical characteristics variables (SDCv), where high number of subjects in study group with age 25-29 years (36%), level of education reach to Institute and college graduate (30%), Occupation was Housewife (66%), Monthly Income was (58%), Residential Area was urban (78%), with Smoking Status was non-smoking (72%). The age at marriage 20-24 years, 42 % of study group period of marriage, while 38% of control group. both group the highest percentage (68%, 88%) respectively were menstruation, number of pregnancy for both groups 52% and 54% and same percentage (88%, 92) for both group were from number of abortion and regarding the para number of baby (58%, 56%) , were both group 48% and 56% respectively period between pregnant, while type of delivery for both groups same percentage (56%). Heredity disease study and control group (86%, 98%), while health problem same percentage of both group 78% and 78%. The results about Knowledge and Practices regarding ANC in pre- test of time show that there were no significant differences at P>0.05 accounted between study and control group regarding all items related to practices of ANC.

    Keywords: Antenatal care; Educational Program; Pregnant women